Platelet Rich Plasma, Glues and Stem Cells

As discussed earlier, the machines that produce PRP are concentrators and separators. The Cytomedix Angel works
by combining a biosensor and a centrifuge, thus separating whole blood into its components: red blood cell concentrate (aka packed red blood cells), plasma, and PRP. We can achieve some interesting effect by recombining some of these components.

The Angel PRP separator from Cytomeidx, Inc.

For example, fibrin glue is a commercially available substance (from the blood bank) that instead can be made right from each individual patient. This is done by mixing the plasma, which contains fibrinogen, with recombinant thrombin, readily available to purchase. PRP can be added to this glue to make a bio-active PRP glue. The glue can be used to encapsulate cells, as is done in the Denovo NT implantation technique.
Work of this type has been published with excellent early success: see Haleem Am et al, the Pittsburgh Orthopedic Journal 2010, p 25

It remains to be seen if preparation of bone marrow derived “stem-like” cells are superior to cartilage cells in this type of treatment. One problem with this technique is that obtaining bone marrow can be painful, and a significant amount of it is needed because the population of stem cells is low, and decreases with age. One option may be to combine bone marrow with peripheral blood and use a machine like the Angel to concentrate the cells. That would potentially create an autologous (from the patient) stem cell concentrate that could be used to regenerate
various tissues.

All of these ideas have in common the concept of using the patients own tissues and proteins for self healing.